Molecular identification of Entamoeba histolytica from diarrhetic patients in Baghdad province, Iraq

Abstract

This study used molecular detection technologies to identify Entamoeba histolytica, the amoeba causing amoebiasis, in people suffering from diarrhoea. We gathered stool samples from one hundred people experiencing symptoms of diarrhoea and classified them according to their age and gender. The findings of an inspection using a direct microscope showed that ninety per cent of the samples had Entamoeba germs. According to the microscopic inspection findings, the infection rate with Entamoeba was much greater in females (86.73%) than in men (70.8%), and this difference was significant at the level of 0.05 for the P value. The investigation also indicated significant infection rates in the age categories of 5-11 years and more than 50 years (respectively 85.23% and 78.81%) but a reduced infection rate in the age group of more than 2 years (43.45%). The nested multiplex polymerase chain reaction, in which the small subunit gene was used, was able to establish that the infection rate for E. histolytica 91 was greater (91 %). The prevalence of Entamoeba histolytica infection is much higher in females (89.23%) than it is in males (82.35%). Both the older age group (>2) and the younger age group (28-48 years) had an infection rate that was one hundred per cent attributable to E. histolytica. Based on gender, age, and the results of nested multiplex PCR, this gave the proportion of people that were infected with Entamoeba histolytica.